A Study of Fixation for Electron Microscopy

Detection of rotavirus by Latex Agglutination Test (Rotales); Comparison with Electron Microscopy and Complement Fixation Test

Microwave-stimulated fixation for electron microscopy using a domestic microwave oven.

We investigated microwave-stimulated fixation of tissues for transmission electron microscopy using a domestic microwave oven operating at a frequency of 2.45 GHz with an output power of 500W. Microwave-stimulated fixation, in 4% glutaraldehyde, of fresh rat kidney, liver, heart and brain tissues was compared to conventional fixation. Human renal biopsies were similarly studied. Electron micros...

Examination of Lung Parenchyma by Electron Microscopy

In this paper many of the principal struc­ture of the Moral Lung were studied with the help of Electron Microscopy. Detailed descrip­tion was given of Granular Pneumocytes, Membranous Pneumocytes, Macrophages, and Alveolar Capillaries. A discussion followed on the form and function of Granular Pneumo­cytes.  Acknowledgements  I am grateful to the Reza Shah Pahlavi Foundation for its generou...

Isolation of Herpetosiphon giganteus and Ultrastructure Analysis by Electron Microscopy

Herpetosiphon giganteus is a filamentous gliding bacterium. Gliding motility is the movement of the cells over surfaces without the aid of flagella. The mechanism responsible for bacterial gliding motility has not been known and there are only a few data on Herpetosiphon giganteus. The aim of this study was to observe the ultrastructure and negative staining of isolated strains of Herpetosiphon...

Dimensional Changes of Articular Cartilage during Immersion-freezing and Freeze-substitution for Scanning Electron Microscopy

In this study, the dimensional changes of articular cartilage during cryofixation (immersion-freezing) followed by freeze-substitution and critical-point-drying or tertiary-butyl alcohol drying were compared with conventional fixation techniques. The cryotechnique resulted in 11% shrinkage of the articular cartilage. During conventional chemical fixation, the sample shrank by 28%. No difference...